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Library of the Biotechnology Department

Cytochrome P450 protocols

Obrazy
Autor
edited by Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano
Miejsce wydania
New York

Wydawca

Rok wydania
2013
Spis treści

Preface . . v
Contributors . . ix

1 Bioluminescent Assays for Cytochrome P450 Enzymes . . 1
2 Simultaneous Determination of Multiple GYP Inhibition Constants using a Cocktail-Probe Approach . . 11
3 High-Throughput Mass Spectrometric Cytochrome P450 Inhibition Screening . . 25
4 The Synthesis, Characterization, and Application of 13C-Methyl Isocyanide as an NMR Probe of Heme Protein Active Sites . . 51
5 High-Throughput Fluorescence Assay for Cytochrome P450 Mechanism-Based Inactivators . . 61
6 Identification of Endogenous Substrates of Orphan Cytochrome P450 Enzymes Through the Use of Untargeted Metabolomics Approaches . . 71
7 Genetic and Mass Spectrometric Tools for Elucidating the Physiological Function(s) of Cytochrome P450 Enzymes from Mycobacterium tuberculosis . . 79
8 An Escherichia coli Expression-Based Approach for Porphyrin Substitution in Heme Proteins . . 95
9 Expression in Escherichia coli of a Cytochrome P450 Enzyme with a Cobalt Protoporphyrin IX Prosthetic Group . . 107
10 Nanodiscs in the Studies of Membrane-Bound Cytochrome P450 Enzymes . . 115
11 Rapid LC-MS Drug Metabolite Profiling Using Bioreactor Particles . . 129
12 Fluorescence-Based Screening of Cytochrome P450 Activities in Intact Cells . . 135
13 Screening for Cytochrome P450 Reactivity with a Reporter Enzyme . . 149
14 High-Throughput Fluorescence Assay of Cytochrome P450 3A4 . . 157
15 Targeted Protein Capture for Analysis of Electrophile-Protem Adducts . . 163
16 DNA Shuffling of Cytochrome P450 Enzymes . . 177
17 Measurement of P450 Difference Spectra Using Intact Cells . . 189
18 DNA Shuffling of Cytochromes P450 for Indigoid Pigment Production . . 205
19 P450 Oxidoreductase: Genotyping, Expression, Purification of Recombinant Protein, and Activity Assessments of Wild-Type and Mutant Protein . . 225
20 LICKED: A Versatile Drop-In Vector for Rapid Generation of Redox-Sclf-Sufficient Cytochromcs P450 . . 239
21 Update on Allele Nomenclature for Human Cytochromes P450 and the Human Cytochrome P450 Allele (CYP-Allele) Nomenclature Database . . 251
22 Simultaneous In Vivo Phenotyping of CYP Enzymes . . 261
23 Detection of Regulatory Polymorphisms: High-Throughput Capillary DNase I Footprinting . . 269
24 Isolation of Mouse Hepatocytes . . 283
25 Highly Efficient SiRNA and Gene Transfer into Hepatocyte-Like HepaRG Cells and Primary Human Hepatocytes: New Means for Drug Metabolism and Toxicity Studies . . 295

Index . . 315

Seria
(Methods in Molecular Biology ; vol. 987)

Biochemistry researcher biographical sketches and research summaries

Obrazy
Autor
James T. Walker and Catherine M. Jones editors
Miejsce wydania
New York
Rok wydania
2013
Spis treści

Preface . . xxiii

Part 1 - Research Biographies . . 1

Chapter 1 Cristina Andres-Lacueva . . 3
Chapter 2 Gry Aletta Bjorlykke . . 9
Chapter 3 Victorio Cadierno . . 11
Chapter 4 Wisit Cheungpasitporn . . 17
Chapter 5 Marcio Jose da Silva . . 21
Chapter 6 Isabella Denis . . 25
Chapter 7 Mark D. Distefano . . 27
Chapter 8 Azza All Kamel El-Sheikh . . 39
Chapter 9 Boris Estrine . . 45
Chapter 10 Tulio Cesar Ferreira . . 47
Chapter 11 Rose Gasnier . . 49
Chapter 12 Suresh Kumar Kailasa . . 51
Chapter 13 Jung-Bum Lee . . 53
Chapter 14 C.-Y. Cynthia Lin . . 57
Chapter 15 Sigrid Mennickent . . 63
Chapter 16 Igho Onakpoya . . 67
Chapter 17 Hugo H. Ortega . . 69
Chapter 18 Paolo Polidori . . 77
Chapter 19 Marzia Salmaso . . 81
Chapter 20 Luas Adriano Santos Do Nascimento . . 83
Chapter 21 Elena Timofeeva . . 85
Chapter 22 Ana Villares . . 87
Chapter 23 Vesna Vucic . . 89
Chapter 24 Paulus S. Wang . . 91
Chapter 25 William R. Ware . . 97
Chapter 26 Anna Watson . . 99
Chapter 27 Lei Xu . . 103
Chapter 28 Oguzhan Yildiz . . 105

Part 2 - Research Summaries on Biochemistry . . 109

Chapter 29 57Fe Mossbauer Spectroscopic and Density Functional Theory (DFT) Study on the Interactions of the Metal Ion with Monosaccharides . . 111
Chapter 30 An Introductory Course on Group Theory and Chemical Applications . . 113
Chapter 31 Aromatic Polyethers Based on Heterocyclic Monomers . . 115
Chapter 32 Bacteriophages and Biofilms: Ecology, Phage Therapy, Plaques . . 117
Chapter 33 Beta-Lactamases . . 119
Chapter 34 Biflavonoids: Occurrence, Structural Features and Bioactivity . . 121
Chapter 35 Binary Aqueous and CO2 Containing Mixtures and the Krichevskii Parameter . . 123
Chapter 36 Bioactive Oligosaccharides: Production, Biological Functions and Potential Commercial Applications . . 125
Chapter 37 Biochemistry and Function of Antifreeze Proteins . . 127
Chapter 38 Biochemistry of Prenatal Alcohol Exposure . . 129
Chapter 39 Biodegradation of Cellulose Fibers . . 131
Chapter 40 Biogenic Amines: Pharmacological, Neurochemical and Molecular Aspects in the CNS . . 133
Chapter 41 Biophysics of DNA-Antibiotic Complexes . . 135
Chapter 42 Borates: Chemical, Pharmaceutical and Pharmacological Aspects . . 137
Chapter 43 Calixarene Complexes with Solvent Molecules . . 139
Chapter 44 Chemical and Biochemical Kinetics: New Perspectives . . 141
Chapter 45 Chemical and Biochemical Reactions: Kinetics and Mechanism . . 143
Chapter 46 Chemical Reactions in Gas, Liquid and Solid Phases: Synthesis, Properties and Application . . 145
Chapter 47 Chemicals and Radioactive Materials and Human Development . . 147
Chapter 48 Chitosan-Based Hydrogels for Tissue Engineering Applications . . 149
Chapter 49 Computational Metabolomics . . 151
Chapter 50 Copolymers in the Preparation of Parenteral Drug Delivery Systems . . 153
Chapter 51 Covalently and Noncovalently Bound Assemblies of Calixarenes . . 155
Chapter 52 Current Status of Chitosan on Dermal/Transdermal Drug Delivery Systems . . 157
Chapter 53 Decreasing Oxidative Stress and Retarding the Aging Process . . 159
Chapter 54 Dipicolinic Acid, its Analogues and Derivatives: Aspects of Their Coordination Chemistry . . 161
Chapter 55 Drug Targeting by Magnetically Responsive Colloids . . 163
Chapter 56 Electrochemical Technology Applied in Treatment of Wastewater and Ground Water . . 165
Chapter 57 Electrospinning Process and Nanofiber Research . . 167
Chapter 58 Folding/Unfolding Kinetics of Lattice Proteins by Applying a Simple Statistical Mechanical Model for Protein Folding . . 169
Chapter 59 Interpolyelectrolyte Complexes of Chitosan . . 171
Chapter 60 Modern Nanochemistry . . 173
Chapter 61 Polymer Assisted Surface Modification by Photons . . 175
Chapter 62 Porous Structure and Adsorption Behaviours of Chitosan . . 177
Chapter 63 Principles of Free Radical Biomedicine. Volume I . . 179
Chapter 64 Protein-Protein Interactions . . 181
Chapter 65 Recent Developments in Bio-Nanocomposites for Biomedical Applications . . 183
Chapter 66 Sampling and Sample Preparation in Analytical Chemistry . . 185
Chapter 67 Soybeans: A Peroxidase Source for the Biotreatment of Effluents . . 187
Chapter 68 Squalene: Current Knowledge and Potential Therapeutical Uses . . 189
Chapter 69 Starch Science Progress . . 191
Chapter 70 Symbiotic Biofilms and Brain Neurochemistry . . 193
Chapter 71 Tannins and Phenolics in Animal Nutrition: Chemistry and Challenges . . 195
Chapter 72 Theoretical Estimation of Acidic Force of Linear Olefins of Cationic Polymerization . . 197
Chapter 73 Alkaloids: Properties, Application and Pharmacological Effects . . 199
Chapter 74 Intraocular Pressure Lowering Effect of Natural Ergot Alkaloids and their Future Applications in Ocular Pharmacology . . 201
Chapter 75 Pilocarpine and Related Alkaloids in Pilocarpus Vahl (Rutaceae) . . 203
Chapter 76 Quaternary Benzo[c]phenantridine Alkaloids: Perspective Fluorescence DNA probes . . 205
Chapter 77 Metabolic Engineering and Alkaloid Producing Pathways: Scopolamine as a Model Case . . 207
Chapter 78 The Effects of Alkaloids on the Feeding of Lepidopteran Larvae . . 209
Chapter 79 Biosynthesis of Chlorinated Alkaloids in Menispermum Dauricum Root Culture . . 211
Chapter 80 Lipids and Cell Function . . 213
Chapter 81 Immune Response Modulation by Targeted Complexes Based on Streptavidin . . 215
Chapter 82 Electrochemical and Optical Biosensors Based on Strept(Avidin)-Biotin Affinity . . 217
Chapter 83 Cardiac (Patho)Physiological Actions of the Classical Mu-, Delta-, and Kappa-Opioid Receptor System . . 219
Chapter 84 Angiotensin Converting Enzyme Inhibitors: A Class of Potent Antihypertensive Agents . . 221
Chapter 85 Protein Superfamilies Based Phylogenomic Analysis of Archaeal Domain . . 223
Chapter 86 Aptamers: The New Biorecognition Element for Proteomic Biosensing . . 225
Chapter 87 Effect on Heavy Oils by Bacteria . . 227
Chapter 88 Effect of Forest Environments on Human Urinary Adrenaline . . 229
Chapter 89 Microarray-Based Assay for Screening Kinase Inhibitors by Biotinylated Gold Nanoparticle Probes . . 231
Chapter 90 ATP at a Crossroads: Cell Life or Death? . . 233
Chapter 91 Bioenergetics of Closed Ecological Systems: Effects of Carbon Sources . . 235
Chapter 92 The Role of Mitochondria in the Glucose Metabolism . . 237
Chapter 93 Mitochondrial Dysfunction Produced by Zn (II) or Selenite: A Comparison with Cd (II) and Ca (II) . . 239
Chapter 94 Extramitochondrial Aerobic Metabolism in Retinal Rod Outer Segment Disks . . 241
Chapter 95 Bioenergetics and Male Infertility: From Basic Science to Clinical Andrology . . 243
Chapter 96 Identification of Specific Mitochondrial Proteins Forming Stable Adducts with 4-Hydroxynonenal within Cardiac Tissue of Type-I Diabetic Animals: Implications for Bioenergetics Dysfunction and Onset of Diabetic Cardiomyopathy . . 245
Chapter 97 Simultaneous Fluorescence and Reflection Confocal Microscopy Study of Living Osteoblast Bioenergetics as a Tool for the Design of Surface Topography of Dental Implants . . 247
Chapter 98 Screening and Studying Photosynthetic Mutants: Basics and Beyond . . 249
Chapter 99 Bioengineering Approaches for Aetiological Research in Severe Pressure Ulcers . . 251
Chapter 100 Ion Beam Bioengineering Research in Thailand . . 253
Chapter 101 Analysis of Shell Structures Applying Triangular Finite Elements . . 255
Chapter 102 Bioengineering of Human Fetal Tissues for Clinical Use . . 257
Chapter 103 Gene Cluster or Operon Design by Ordered Gene Assembly in Bacillus Subtilis (OGAB) Method . . 259
Chapter 104 Bioengineering of Glass-Ceramics and Ceramics for Dental Restoration . . 261
Chapter 105 Development of a Diagnostic System for Osteoarthritis Using a Photoacoustic Measurement Method and Time-Resolved Auto-Fluorescence . . 263
Chapter 106 Biotechnology and Agriculture . . 265
Chapter 107 Calcium Orthophosphate-Based Biocomposites and Hybrid Biomaterials . . 267
Chapter 108 Calcium Orthophosphate Cements and Concretes . . 269
Chapter 109 Hydrogels in Biology and Medicine . . 271
Chapter 110 Biomaterials in Dentistry and Medicine . . 273
Chapter 111 Biomaterials in Blood-Contacting Devices: Complications and Solutions . . 275
Chapter 112 Biocompatibility of Dental and Medical Materials . . 277
Chapter 113 Enhancing Remineralization of Subsurface Enamel Lesions with Functionalized (β-TCP) . . 279
Chapter 114 Translation of Emerging Hydrogel Therapies: The Role of Metrology . . 281
Chapter 115 Metal Ion Release from the Base Co-Cr-Mo, Ni-Cr, and Noble Au-Pt Dental Alloy into the Buffered Solutions of Different Composition and pH Value . . 283
Chapter 116 Co-Cr-Mo Alloy Surface Features and Composition Prior and after Mechanical Polishing and Corrosion in Fluids Simulating Oral Conditions . . 285
Chapter 117 Intracellular Delivery of Gold Nanoparticles: Applications in Nanomedicine . . 287
Chapter 118 A Commentary on Neural Tissue Engineering in the Central Nervous System — Interfacing a Lesion . . 289
Chapter 119 Biosurfactants and Their Uses in the Petroleum Industry and Hydrocarbon Pollution Remediation . . 291
Chapter 120 Detergents in Molecular Biology: DNA Extraction and Purification . . 293
Chapter 121 Carbohydrases in Detergents . . 295
Chapter 122 The Influence of Different Detergents on the Preservation of Discrete Membrane Microdomains: A Comparative Ultrastructural Study on Whole-Mounted Colorectal Cancer Cells . . 297
Chapter 123 -Sulfo Fatty Methyl Ester Sulfonates (Φ-MES): A New Anionic Surfactant . . 299
Chapter 124 Acacia Caven (Mol.) Molina Pollen Proteases: Application to the Peptide Synthesis and to Laundry Detergents . . 301
Chapter 125 Pharmacogenetics of Glucocorticoid Response . . 303
Chapter 126 The Glucocorticoid Receptor Signaling in Cancer and Inflammation . . 305
Chapter 127 Glucocorticoids - Age Specific Impact on Mitochondrial Energy Homeostasis . . 307
Chapter 128 Glucocorticoids, Stress and Cancer: New Insights in Immune System and Tumour Cell Regulations . . 309
Chapter 129 Hypothalamic Obesity: A Pivotal Role of Endogenous Glucocorticoid . . 311
Chapter 130 The Role of Nitric Oxide - Redox Imbalance in Glucocorticoid-Induced Hypertension . . 313
Chapter 131 Glucocorticoids Mediate Drug Resistance: Mechanisms and its Clinical Impact . . 315
Chapter 132 Glucocorticoids in Perioperative Care . . 317
Chapter 133 Glucocorticoid-Mediated Regulation of Tight Junctions in Brain Vascular Endothelium . . 319
Chapter 134 Glucocorticoid Therapy for Cochlear Injuries: Pharmacology and Evidence from Animal Experiments . . 321
Chapter 135 Expert Commentary: Glucocorticoids and Exercise Performance in Humans: Current Status . . 323
Chapter 136 Glucocorticoids, Thymus Function and Sex Hormone in Human Body Growing . . 325
Chapter 137 Nutritional Factors and Osteoporosis Prevention . . 327
Chapter 138 Regulation of Phosphate Transport in Epithelia . . 329
Chapter 139 Methods for the Extraction of Metabolites from Plant Tissues . . 331
Chapter 140 Application of 'Omics' Technologies for Improvement of Meat Quality . . 333
Chapter 141 Role of Chaperones in Dystrophic and Senescent Skeletal Muscle Fibres . . 335
Chapter 142 Amino Acid Composition of Some Aquaculture Fauna Resources in Nigeria . . 337
Chapter 143 The Biochemistry of Isoelectric Processing and Nutritional Quality of Proteins and Lipids Recovered with This Technique . . 339
Chapter 144 Nutritional Biochemistry of Curcumin (Diferuloylmethane) and a Review of Its Biological Actions on Articular Chondrocytes . . 341
Chapter 145 Effect of Creatine Applied as Food Supplement on Human Metabolism . . 343
Chapter 146 Production, Properties and Stability of Chicken Meat Protein Hydrolysate Powder . . 345
Chapter 147 Alpha-Galactosidase: A Food and Feed Enzyme . . 347
Chapter 148 Foods of Plant Origin as Source of Nitric Oxide Production Inhibitors . . 349
Chapter 149 Application of Plant-Derived Food Lectins in Proteoglycomics and Immunomodulation . . 351
Chapter 150 Biochemical and Proteomic Profiling of Key Metabolic Enzymes in Aging Skeletal Muscle . . 353
Chapter 151 Inhibitory Mechanism of Longer Chain Fatty Acids on Mammalian DNA Polymerase β Activity . . 355
Chapter 152 Endorhizal Fungi Associated with Vascular Plants on Truelove Lowland, Devon Island, Nunavut, Canadian High Arctic . . 357
Chapter 153 Involvement of the Mitochondrial ATP-Sensitive Potassium Channel in the Beneficial Effects of Fasting on the Ischemic-Reperfused Rat Heart . . 359
Chapter 154 Application of Artificial Neural Network for Predicting Physicochemical Properties of Pressure-Induced Gel from Industrial Milk Whey Proteins . . 361
Chapter 155 Isoflavones: a Race after the Rescue of the Ageing Hippocampus . . 363
Chapter 156 Prevention of Cardiovascular Diseases by Soy Protein and Isoflavones . . 365
Chapter 157 Effects of Processing on Isoflavone Content and Profile in Foodstuffs: A Review . . 367
Chapter 158 Dietary Isoflavones and Intestinal Microbiota: Metabolism and Transformation into Bioactive Compounds . . 369
Chapter 159 Bioactive Isoflavones: Implications in Cancer Treatment and Prevention . . 371
Chapter 160 Comparative in Vitro Effects of Soybean Isoflavones and Resveratrol on Human Osteoblastic Cells . . 373
Chapter 161 Health Effects of Dioscorea Bulbifera of Aerial-Potato (Yam) . . 375
Chapter 162 Effects of the Processing Techniques on Isoflavone Profiles . . 377
Chapter 163 Structural Studies and Mechanisms of Isoflavonoid Biosynthesis . . 379
Chapter 164 Photophysics of Genistein and Biochanin a Isoflavones: Solvent Cage and Concentration Effects Studied by UV Visible Spectroscopy . . 381
Chapter 165 Soy Isoflavone Genistein in Cancer Chemoprevention . . 383
Chapter 166 Isoflavones in Leguminosae . . 385
Chapter 167 Diffuse Neutron Scattering Study of Correlation Effects among Thermal Displacements of Atoms . . 387
Chapter 168 Small Angle Scattering Analysis of Virus-like Particles for Biomedical Diagnostic Assays . . 391
Chapter 169 Small Angles Neutrons Scattering Application in Biological Materials . . 391
Chapter 170 Neutron Scattering in Skin Research . . 393
Chapter 171 Precipitate Analysis of Microalloyed Steels using Small Angle Neutron Scattering . . 395
Chapter 172 Small Angle Neutron Scattering from Proteinsin Solution . . 397
Chapter 173 Neutron and X-Ray Diffraction Studies of Nanoparticles Confined within Porous Media . . 399
Chapter 174 Methods in Protein Structure and Stability Analysis: Conformational Stability, Size, Shape and Surface of Protein Molecules . . 401
Chapter 175 Neutron Scattering from Solutions of Biological Macromolecules . . 403
Chapter 176 Elastic, Quasi Elastic and Inelastic Neutron Scattering Studies on Hydrogen-Bonded Systems of Biophysical Interest . . 405
Chapter 177 Milk Oligosaccharides . .407
Chapter 178 Prebiotic Oligosaccharides: Origins and Production, Health Benefits and Commercial Applications . . 409
Chapter 179 Glycoside Hydrolases from Hyperthermophiles: Structure, Function and Exploitation in Oligosaccharide Synthesis . . 411
Chapter 180 Enzymatic Synthesis of Linear, Cyclic and Complex Type Oligosaccharides . . 413
Chapter 181 Precursor N-Linked Oligosaccharides as Codes for Glycoprotein Folding Status . . 415
Chapter 182 Oligosaccharides from Sucrose via Glycansucrases . . 417
Chapter 183 Electrospray Mass Spectrometry of Oligosaccharides of Plant Origin . . 419
Chapter 184 Biologically Active Oligosaccharide Functions in Plant Cell: Updates and Prospects . . 421
Chapter 185 The Mannan Oligosaccharides in Aquaculture . . 423
Chapter 186 Facile Synthesis of Unnatural Oligosaccharides by Phosphorylase-catalyzed Enzymatic Glycosylations Using New Glycosyl Donors . . 425
Chapter 187 Oligosaccharides: Sources, Properties and Applications . . 427
Chapter 188 Initiators for Anionic Polymerization: Old and New Developments . . 429
Chapter 189 Advanced Organocobalt Initiators of Radical Polymerization: Current State and Potential Applications . . 431
Chapter 190 Surface-Initiated ATRP as a Versatile Method to Hybrid Materials . . 433
Chapter 191 Citric Acid: An Emerging Substrate for the Formation of Biopolymers . . 435
Chapter 192 Functional Initiators in Radical Polymerization . . 437
Chapter 193 Assessing the Effect of Selected Photoinitiators on the Properties of Photopolymerised Temperature Sensitive Hydrogels . . 439
Chapter 194 Functional Oligomeric Azo-Initiators for Grafting of Polymers on Clay Surface . . 441
Chapter 195 Photoinitiating Systems Based on Unusual Radicals . . 443
Chapter 196 Role of Monocationic and Bicationic Initiators on the Grafting of Polymer Chains 'from' the Clay Surface . . 445
Chapter 197 Designing Non-Classical Inclusion Bodies . . 447
Chapter 198 Extraribosomal Functions of Ribosomal Protein SI9" in Monograph Ribosomal Proteins . . 449
Chapter 199 Order-Disorder Transitions in Ribosome Assembly . . 451
Chapter 200 Disruption of Ribosomal Protein Gene Expression in Eukaryotes: A Class of Mutations Causing Similar Global Growth Defects with Cellular Variations . . 453
Chapter 201 Ancestral Protein Reconstruction as a New Field in Protein Engineering . . 455
Chapter 202 Ribosomal Proteins in Important Tropical Diseases . . 457
Chapter 203 Three Challenges in Post-Proteomics Time . . 459
Chapter 204 Self-similar Property of Ribosomal Proteins . . 461
Chapter 205 Synthesizing Mammalian Cell Adhesive Surfaces using Self-Assembling Fibronectin Domains . . 463
Chapter 206 Halophilic Adaptations of Proteins . . 465
Chapter 207 Alkaline Adaptation of Proteins . . 467
Chapter 208 Preface to the Inaugural Issue of the Journal of Biochemistry and Molecular Biology in the Post-Genomic Era . . 469
Chapter 209 Biochemistry and Molecular Biology in Hong Kong: Past and Future . . 473
Chapter 210 Calpains-Mediated PTEN Cleavage in Excitotoxic Neuronal Death . . 477
Chapter 211 NUDCD2 Is Overexpressed in Head and Neck Squamous Cell Carcinoma and Is Involved in Nuclear Division . . 479
Chapter 212 Genotype Analyses Using SNP (Using MALDI-TOF Mass Spectrometry) and STR (Microsatellite) Markers in the Determination of Zygosity Status of Chinese Twins . . 481
Chapter 213 The Dyslexia Candidate Gene Kiaa0319L Encodes N-Glycosylated Isoforms That Form Homo-Dimers . . 483
Chapter 214 Identification of Feed Forward Loops Composed of MicroRNAs and Transcription Factors in Arabidopsis . . 485
Chapter 215 Sanfilippo Syndrome: Identification and Removal of a Cryptic Splice Site in Alpha-N-Acetylglucosaminidase cDNA for Potential Improved Expression and Enzyme Replacement Therapy . . 487
Chapter 216 Conference Report of 2010 Inter-University Post-Graduate Symposium . . 489
Chapter 217 Launching Theme-Based Research in Hong Kong . . 491
Chapter 218 Chaos Theory in Astrophysics, Nature, Genetics, and Human Cancer . . 493
Chapter 219 Structural and Functional Studies of Zonula Occludens-1 . . 495
Chapter 220 Global Gene Expression Changes during Chondrocyte Adaptation to ER Stress . . 497
Chapter 221 Genetic Analysis of Zebrafish Hematopoiesis-Defective Mutant Thy-193 . . 499
Chapter 222 Structural and Functional Analysis of Influenza H5N1 Nucleoprotein Reveals Amino Acids That Are Crucial for Oligomerization, RNA Binding and Ribonucleoprotein Activities . . 501
Chapter 223 Structural Basis of the Organization of the USH1 Interactome . . 503

Seria
(Nova Biomedical. Biochemistry Research Trends)

New developments in chromatin research

Obrazy
Autor
Neil M. Simpson and Valerie J. Stewart editors
Miejsce wydania
New York
Rok wydania
2012
Spis treści

Preface . . vii

Chapter 1 The Varied Functions of Aurora Kinases A and B in Mitosis and Carcinogenesis . . 1
Chapter 2 New Developments in Chromatin Research: An Epigenetic Perspective . . 29
Chapter 3 Sperm Chromatin Integrity, DNA Fragmentation and Male Fertility . . 59
Chapter 4 The Chromatin State of Pluripotent Stem Cells . . 83
Chapter 5 MITF Meets Chromatin in Melanoma . . 105
Chapter 6 The State of Chromatin as an Integrative Indicator of Cell Stress . . 123
Chapter 7 A Genomics Approach to Analysing DNA Damage and Its Repair throughout Entire Genomes . . 145
Chapter 8 Recent Patent on Revolver-2: A Novel Transposon-Like Gene Useful for Chromosome Tags of Rye . . 161
Chapter 9 The Removal of DNA Damage Is Promoted by the Yeast Global Genome Nucleotide Excision Repir Factor Rad16 . . 177
Chapter 10 Chromatin Condensation in Infertile Sperm . . 195
Chapter 11 Glucocorticoid-Induced Chromatin Remodeling: A Novel Molecular Mechanism of Traumatic Stress . . 211

Index . . 223

Seria
(Nova Biomedical. Cell Biology Research Progress. Genetics, Research and Issues)

Potassium channels : types, structure and blockers

Obrazy
Autor
Danielle S. Fonseca editor
Miejsce wydania
New York
Rok wydania
2012
Spis treści

Preface . . vii

Chapter I Potassium Channels and Vascular Tone: Structure, Regulation and Function . . 1
Chapter II The Contribution of Lymphocyte Potassium Channels to the Perinatal Regulation of the Immune Response in Mother and Newborn . . 49
Chapter III Mitochondrial Potassium Channel: Features and Physiological Participation . . 69
Chapter IV Slow Potassium Channel Dysfunction in Amyotrophic Lateral Sclerosis and Its In Vivo Evaluation by Threshold Tracking . . 83
Chapter V Plant Mitochondrial Potassium: Channel or Channels? . . 97
Chapter VI Involvement of the Mitochondrial ATP-Sensitive Potassium Channel in the Beneficial Effects of Fasting on the Ischemic-Reperfused Rat Heart . . 119

Index . . 135

Seria
(Nova Biomedical. Cell Biology Research Progress)

Genetically encoded functional indicators

Obrazy
Autor
edited by Jean-René Martin
Miejsce wydania
New York

Wydawca

Rok wydania
2012
Spis treści

Preface to the Series . . v
Preface . . vii
Contributors . . xi

1. In Vivo Functional Brain Imaging Using a Genetically Encoded Ca2+-Sensitive Bioluminescence Reporter, GFP-Aequorin . . 1
2. Ca2+ Imaging in Brain Slices Using Bioluminescent Reporters . . 27
3. Calcium Imaging of Neural Activity in the Olfactory System of Drosophila . . 43
4. Functional Imaging of Antennal Lobe Neurons in Drosophila with Synapto-pHluorin . . 71
5. Performing Electrophysiology and Two-Photon Calcium Imaging in the Adult Drosophila Central Brain During Walking Behavior . . 83
6. In Vivo Optical Recording of Brain Interncuron Activities from a Drosophila Male on a Treadmill . . 103
7. Two-Photon Imaging of Population Activity with Genetically Encoded Calcium Indicators in Living Flies . . 113
8. Engineering and Application of Genetically Encoded Calcium Indicators . . 125
9. Imaging cAMP Dynamics in the Drosophila Brain with the Genetically Encoded Sensor Epac1-Camps . . 149

Index . . 169

Seria
(Springer Protocols. Neuromethods ; 72)

Interferons : characterization, mechanism of action and clinical applications

Obrazy
Autor
Joe H. Thomas and Adrian Roberts editors
Miejsce wydania
New York
Rok wydania
2012
Spis treści

Preface . . vii
Chapter I Interferon Regulatory Factors in Immune Cell Development and Host Response to Infections . . 1
Chapter II Interferon-Gamma, Oligodendrocyte Injury and Inflammatory Demyelination . . 67
Chapter III Interferons and Their Effect on NK Cells Activity in HIV Infection and Tumour Cells Development . . 83
Chapter IV Interferons, Serine Proteases and Protease-Activated Receptors: Enemies or Partners? . . 95
Chapter V The Roles of IFN- y in Esophageal Squamous Cell Carcinoma . . 107
Chapter VI Interferons in Multiple Sclerosis: Clinical Applications and Possible Mechanisms of Actions . . 119
Chapter VII Dynamics of Lymphocyte Subpopulations in Patients with Chronic Hepatitis C Virus Infection During Pegylated Interferon-Alpha2a and Ribavirin Treatment . . 129
Chapter VIII Co-Administration of Gamma Interferon Gene with DNA Vaccine Expressing Duck Hepatitis B Virus (DHBV) Proteins Enhances Therapeutic Efficacy of DNA-Based Immunization in Chronic Virus Carriers . . 135
Chapter IX Rabies Virus Evasion of the Interferon System . . 163
Index . . 163

Seria
(Nova Biomedical. Protein Biochemistry, Synthesis, Structure and Cellular Functions)