Preface to the Series . . v
Preface . . vii
Contributors . . xi

1. In Vivo Functional Brain Imaging Using a Genetically Encoded Ca2+-Sensitive Bioluminescence Reporter, GFP-Aequorin . . 1
2. Ca2+ Imaging in Brain Slices Using Bioluminescent Reporters . . 27
3. Calcium Imaging of Neural Activity in the Olfactory System of Drosophila . . 43
4. Functional Imaging of Antennal Lobe Neurons in Drosophila with Synapto-pHluorin . . 71
5. Performing Electrophysiology and Two-Photon Calcium Imaging in the Adult Drosophila Central Brain During Walking Behavior . . 83
6. In Vivo Optical Recording of Brain Interncuron Activities from a Drosophila Male on a Treadmill . . 103
7. Two-Photon Imaging of Population Activity with Genetically Encoded Calcium Indicators in Living Flies . . 113
8. Engineering and Application of Genetically Encoded Calcium Indicators . . 125
9. Imaging cAMP Dynamics in the Drosophila Brain with the Genetically Encoded Sensor Epac1-Camps . . 149

Index . . 169